Inducers of mammalian cell differentiation stimulate dome formation in a differentiated kidney epithelial cell line (MDCK).
نویسندگان
چکیده
منابع مشابه
Gangliosides stimulate dome formation in cultured canine kidney epithelial cell line (MDCK).
The effect of exogenous gangliosides on the occurrence of domes in MDCK cell cultures was investigated in view of the involvement of both dome formation and gangliosides in cell growth, differentiation and transepithelial transport. Dome formation was increased by gangliosides in medium free of fetal calf serum. Among the gangliosides tested, GM3 and GD3 isolated from porcine kidney were most a...
متن کاملRetention of differentiated properties in an established dog kidney epithelial cell line (MDCK)
Madin-Darby canine kidney (MDCK) cells grown in tissue culture have the morphological properties of distal tubular epithelial cells, form tight junctions, and lack several proximal tubular enzyme markers. Adenylate cyclase in these cells was stimulated by vasopressin, oxytocin, prostaglandins E1 and E2, glucagon, and cholera toxin. Hormone-stimulated adenylate cyclase activity in isolated membr...
متن کاملThe MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule
Monoclonal antibodies were prepared against the Madin-Darby canine kidney (MDCK) cell line to identify epithelial cell surface macromolecules involved in renal function. Lymphocyte hybrids were generated by fusing P3U-1 myeloma cells with spleen cells from a C3H mouse immunized with MDCK cells. Hybridomas secreting anti-MDCK antibodies were obtained and clonal lines isolated in soft agarose. We...
متن کاملGrowth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate subs...
متن کاملDome formation by a retrovirus-induced lung adenocarcinoma cell line.
A cell line has been established from an alveologenic lung adenocarcinoma that develops in newborn NFS/N mice after i.p. inoculation with a carcinoma-inducing retrovirus. A clonal derivative of this cell line, 3041, forms adenocarcinomas when injected into syngeneic weanling mice and in culture retains several properties typical of alveologenic type 1 cells. These include morphological details,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.3.1323